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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
18/12/2020 |
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Actualizado : |
18/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
COPPOLA, N.; FREIRE, B.; UMPIÉRREZ, A.; CORDEIRO, N.F.; AVILA. P.; TRENCHI, G.; CASTRO, G.; CASAUX, M.L.; FRAGA, M.; ZUNINO, P.; BADO, I.; VIGNOLI, R. |
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Afiliación : |
NADIA COPPOLA, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; BIBIANA FREIRE, Unidad Académica Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.; ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; NICOLÁS F. CORDEIRO, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; PABLO AVILA, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; GUSTAVO TRENCHI, Veterinario de Libre Ejercicio, Montevideo, Uruguay.; GUSTAVO CASTRO, Unidad Académica Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; INÉS BADO, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Transferable Resistance to Highest Priority Critically Important Antibiotics for Human Health in Escherichia coli Strains Obtained From Livestock Feces in Uruguay. (Brief Research Report Article). |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Frontiers in Veterinary Science, 19 November 2020, Volume 7, Article number 588919. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2020.588919 |
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DOI : |
10.3389/fvets.2020.588919 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 29 July 2020/ Accepted: 19 October 2020/Published: 19 November 2020. Correspondence: Rafael Vignoli, rvignoli@higiene.edu.uy; Inés Bado, ibado@higiene.edu.uy |
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Contenido : |
Abstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in which the search for transferable resistance to highest priority critically important antibiotics for human health is carried out in chickens and pigs chains of production animals in Uruguay. MenosAbstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in wh... Presentar Todo |
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Palabras claves : |
CMY-2; CTX-M-8; E. COLI; ESBL; ESCHERICHIA COLI; MCR-1; PLATAFORMA SALUD ANIMAL; POULTRY; QNRB19; SWINE. |
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Thesagro : |
AVES DE CORRAL; CERDO. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03119nam a2200409 a 4500
001 1061590
005 2020-12-18
008 2020 bl uuuu u0uu1 u #d
024 7 $a10.3389/fvets.2020.588919$2DOI
100 1 $aCOPPOLA, N.
245 $aTransferable Resistance to Highest Priority Critically Important Antibiotics for Human Health in Escherichia coli Strains Obtained From Livestock Feces in Uruguay. (Brief Research Report Article).$h[electronic resource]
260 $aFrontiers in Veterinary Science, 19 November 2020, Volume 7, Article number 588919. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2020.588919$c2020
500 $aArticle history: Received: 29 July 2020/ Accepted: 19 October 2020/Published: 19 November 2020. Correspondence: Rafael Vignoli, rvignoli@higiene.edu.uy; Inés Bado, ibado@higiene.edu.uy
520 $aAbstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in which the search for transferable resistance to highest priority critically important antibiotics for human health is carried out in chickens and pigs chains of production animals in Uruguay.
650 $aAVES DE CORRAL
650 $aCERDO
653 $aCMY-2
653 $aCTX-M-8
653 $aE. COLI
653 $aESBL
653 $aESCHERICHIA COLI
653 $aMCR-1
653 $aPLATAFORMA SALUD ANIMAL
653 $aPOULTRY
653 $aQNRB19
653 $aSWINE
700 1 $aFREIRE, B.
700 1 $aUMPIÉRREZ, A.
700 1 $aCORDEIRO, N.F.
700 1 $aAVILA. P.
700 1 $aTRENCHI, G.
700 1 $aCASTRO, G.
700 1 $aCASAUX, M.L.
700 1 $aFRAGA, M.
700 1 $aZUNINO, P.
700 1 $aBADO, I.
700 1 $aVIGNOLI, R.
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| Registros recuperados : 2 | |
| 1. |  | GADEA, M.P.; VARELA, G.; BALSEIRO, V.; GONZÁLEZ, S.; MOTA, M.I.; GONZÁLEZ, G.; PARDO, L.; CATTÁNEO, M.; CORDEIRO, N.; SCHELOTTO, F.; GADEA, M.P. Detección y caracterización de Escherichia coli productor de toxina Shiga a partir de casos clínicos de alimentos y de animales en Uruguay. Carnes y Alimentos, 2009, v. 10, no. 32, p. 34-48.| Biblioteca(s): INIA Tacuarembó. |
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| 2. | | COPPOLA, N.; FREIRE, B.; UMPIÉRREZ, A.; CORDEIRO, N.F.; AVILA. P.; TRENCHI, G.; CASTRO, G.; CASAUX, M.L.; FRAGA, M.; ZUNINO, P.; BADO, I.; VIGNOLI, R. Transferable Resistance to Highest Priority Critically Important Antibiotics for Human Health in Escherichia coli Strains Obtained From Livestock Feces in Uruguay. (Brief Research Report Article). Frontiers in Veterinary Science, 19 November 2020, Volume 7, Article number 588919. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2020.588919 Article history: Received: 29 July 2020/ Accepted: 19 October 2020/Published: 19 November 2020. Correspondence: Rafael Vignoli, rvignoli@higiene.edu.uy; Inés Bado, ibado@higiene.edu.uy| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 2 | |
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